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Laboratory diagnostics makes it possible not only to differentiate infectious enterotoxemia from similarly occurring diseases, but also to determine the type of pathogens, which is of decisive importance in the fight against the disease.

Diagnosis and differential diagnosis. The diagnosis of infectious enterotoxemia is made on the basis of a comprehensive study: epizootological data, results of a clinical examination, pathoanatomical autopsy and laboratory tests.

Pathological material must be taken no later than 3. 4 hours after the death of the animal. The following are sent to the laboratory: corpses of lambs, altered segments of the small intestine with contents, altered sections of baricitinib pills, parenchymal organs, lymph nodes, subcutaneous tissue infiltrate, tubular bone, exudate from the abdominal cavity. In the warm season, the pathological material is conserved.

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In both cases, typing (determination of the type of toxin) is necessary in pathological material or culture in the neutralization reaction (RN) in mice according to the following scheme (Table 1.11).

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Thus, the final diagnosis of enterotoxemia is considered established when a toxin is detected in the filtrate of the contents of the small intestine and its type is determined - the isolation of the culture of C. perfringens that produces the toxin and the establishment of its type in the pH.

If a toxin is detected, the culture may not be isolated. The presence of a specific toxin and the establishment of its type means the final diagnosis of infectious enterotoxemia.

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Laboratory diagnostics makes it possible not only to differentiate infectious enterotoxemia from similarly occurring diseases, but also to determine the type of pathogens, which is of decisive importance in the fight against the disease.

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To make a diagnosis of enterotoxemia, it is necessary to detect a toxin (determination of toxicity) in the material or isolate a pure culture by bacteriological examination.

Determination of the type of toxin C. Perfringens.